RESUMO
ABSTRACT In Cuba, newborn screening (NBS) for cystic fibrosis (CF) was introduced in January 2019. The results from the first three years of the CF NBS program are presented. An IRT/IRT protocol was followed using a cut-off value of 50 ng/mL. In this period 281,717 neonates were screened, 2,197 samples had increased IRT values, and a second sample was necessary (recall rate=0.78%). In 686 (0.24%) neonates, IRT was still elevated, and they were referred for clinical evaluation. Twenty-one children were confirmed by sweat test and molecular biology. Eighteen newborns presented variant F508del. A false negative case was reported. Demographic data of 32,764 neonates were collected. The average age of sampling was six days with results available at 11 days of life, but 1.7% of the samples were collected 20 days after birth. The mean IRT value was 12.7±11.7 ng/mL (ranging 0-283 ng/mL) with a calculated 98.5 percentile value of 42.4 ng/mL. On average, the samples were processed five days after collection and two days after they were received at the laboratory. Although CF NBS program in Cuba is just beginning, it can be predicted that CF will be one of the most frequent inherited-metabolic diseases in the Cuban population.
RESUMO
En el seguimiento de las personas afectadas con el VIH-1 resulta de gran utilidad el empleo de sistemas para detectar la presencia de proteina de 24 KD y los niveles de anticuerpos a esta; para el desarrollo de estos sistemas es necesario contar con esta proteina purificada. El presente trabajo describe la purificacion de p24 a partir de lisado rival semipurificado sobre gradiente de sacarosa, empleando cromatografia de inmunoafinidad con anticuerpos monoclonales acoplados a Sefarosa 4B activada con bromuro de cianogeno. Para la caracterizacion del producto purificado se utilizo electroforesis en gel de poliacrilamida con tincion de plata e inmunoelectrotransferencia. Como resultado de este trabajo se obtuvo p24 util para ser empleada en el desarrollo de medios diagnosticos y anticuerpos monoclonales